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Hey, does anyone know any effective way to separate virus from exosomes fraction? my problem with the separation of the EVs in this system is the fact that the virus that we work with particles...

06 December 2020 4,765 2 View

Number of cells for extacellular vesicles extraction?

hello! I want to extract vesicles from MDA MB 231 cell ligne, culture in serum free media for 48h, would you help me with th number of cells required to have the vesicles? thank you

03 December 2020 6,488 2 View

What are the XRD analysis conditions of the cement, sand and Limestone ?

I am a Phd student and I need to do XRD analysis on my pouder samples namely ( cement- sand- limestone and calcined clay). I was asked asked to specify the analysis conditions: -the nature of...

07 October 2020 5,737 6 View

Can anyone recommend me some articles about Fire endurance of wood concrete?

Are there documentations about Fire endurance of wood or wood shavings concrete? The literature is not very clear about this .

12 August 2020 8,203 3 View

Is it possible to use plant extract as natural sterilizer?

For economic reasons, sterilzation against covid_19 is very necessary, so is it possible to use plant extract?

11 May 2020 2,674 14 View

Which statistical analysis is more appropriate for my study?

I am trying to find the relationship between the use of defence mechanisms in early adulthood as predictors of neuroticism. Those who use more defence mechanisms will score higher on the...

14 April 2020 3,353 7 View

How do I carry out performance evaluation on a sorting machine?

I design a prototype for sorting tomatoes based on size and colours. The prototype will sort big red, big green, small green and small red

12 March 2020 2,386 3 View

Preparation of the mix for Standard PCR (without using a kit)?

Hi, Please anyone already prepared the mix for Standard PCR? But without using a commercial kit. It is for staphylococcal enterotoxins genes. I am looking for the concentrations of each...

08 February 2020 4,487 6 View

How to deal with mental health issues?

I feel communication helps in coping such issues to some level.

16 August 2019 7,405 31 View

Course -Preparation advices !?

Dear colleagues, As I am new in the domain of teaching courses, so it takes too much of time to prepare my course, saying for example: cryptography course, web development,..etc,... So, my...

30 May 2019 6,236 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

What could be the possible reason for the drastic variation in Qmax value for same adsorbent onto two different cationic dyes itself?

The adsorption capacity of the rice husk biochar was varying drastically with two cationic dyes namely Basic Blue 41 and Basic Red 09? What could be the possible reason behind this?

30 January 2021 1,420 3 View

After cutting bands having low molecular weight peptides obtained from SDS-PAGE,is it possible to elute these peptide(s) without digesting in trypsin?

Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...

30 January 2021 5,482 3 View