User Guide for Perkin-Elmer LS 55 Luminescence Spectrometer?

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Luciferase assay lysis?

Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...

13 June 2024 9,936 5 View

How could I analyze the results of caspase-glo 3/7 assay?

I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...

02 June 2024 7,833 2 View

Any low-cost mid-infrared spectrometer?

If we build up a mid-infrared laser system and want to measure its spectra, where can I find the lowest-cost mid-infrared spectrometer, ranging from 2 to 20 μm。 Any recommendation? Thanks!

26 May 2024 8,114 1 View

Does anyone have Atomic Absorption Spectrometer TAS- 990 manual?

Atomic Absorption Spectrometer

22 May 2024 946 1 View

Orbitrap Astral Mass Spectrometer: applications?

Hi everyone, I'm a PhD student at CAST, Analytical Biochemistry and Proteomics Research Unit, University, "G. d'Annunzio" of Chieti-Pescara. We currently use the Thermo Fisher Orbitrap Fusion...

10 April 2024 9,346 0 View

NIR/SWIR microspectrophotometer?

Good afternoon, I was wondering if anyone in UK has a NIR/SWIR microspectrophotometer or a spectrometer for transmission measurements between 850 nm and 2000 nm? Our samples are on a 2 cm x 2cm...

09 April 2024 3,761 1 View

Can someone explain the procedure for Calculating Judd Ofelt Parameters?

I'm working on a project related to Luminescence Properties of Rare Earth Glasses (not Europium). I have read some articles related to Judd Ofelt Parameters, but I couldn't able to understand the...

26 March 2024 2,287 0 View

What factors contribute to an incorrect CSA ratio for CD calibration?

Hello everyone! I am new to circular dichroism spectrometry, and am running into a problem with our CD spectrometer (Aviv 202). One calibration that is recommended is checking the ratio of the...

26 March 2024 1,797 1 View

My western blot exposures are weak and taking a long time to develop why?

My transfer buffer Ph is 8.7 but should be a 8.3 is this causing my exposures to be off also my bands look smeared. i am trying to troubleshoot and figure out why my chemiluminescence western blot...

12 March 2024 7,433 1 View

Manijeh Rahimi

Dear Masoud,

I don't have a phf of that but I have a book. can you tell me what part of it you want?

Muayad Masoud

Thank you (@ Manijeh Rahimi) very much!

I got it in pdf format from Perkin Elmer.

Jean Rene Grezes

According to your previous answer, please insert:

Perkin-Elmer LS 55 Luminescence Spectrometer ?

in Google and you will find a lot of interesting infos and hints about it and your research.

JRG