Is it possible to have two luminescence bands of the same peak position but with different intensities in single spectrum??

Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...

26 February 2021 4,963 5 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

How can I analysis a low-concentration organic matter in NaOH using fluorescence spectrometer or LC-OCD?

I want to analysis components of organic matter in NaOH using fluorescence spectrometer or LC-OCD. For example, TOC result is 3 mg/L, it is very low. So I want to concentrate or extract the...

23 November 2020 4,365 1 View

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17 November 2020 6,197 5 View

Why use STOP solution in ELISA?

At the last step for data analysis, we always use a STOP solution to stop the TMB oxidized by HRP. Why do we have to stop the reaction? Couldn't we measure the color wavelength of...

17 November 2020 5,177 3 View

Some ideas about contamination from Ca 2p always appearing and, therefore, overlaping with Pd3d in a SPECS spectrometer?

Dear colleagues, In our XPS instrument, most of the time when we acquire the Pd3d region (for a wide range of materials containing Pd) it overlaps with a strong signal from Ca2p. Nonetheless,...

10 November 2020 4,487 4 View

What would cause Cell Titre Glo assay wells to luminesce less than the media only wells?

Hi all, I'm doing a bunch of Cell Titre Glo assays to assess the effects of several inhibitors on 2 cell lines. Problem is, for some of the wells they seem to be luminescing less than the...

30 October 2020 1,513 2 View

What can be the cause of a baseline drift in NIR spectrometry?

I have a dataset including spectra collected with a handled NIR spectrometer (SCiO) over several months. I observe a baseline drift that is consistent with the evolution of the measurement date....

18 October 2020 9,062 3 View

How I can find the quantum efffcincy from the measured and calculated decay time when the measured decay time is greater than the calcualted decay ?

I am working on the luminescence properties of Nd3+ ions doped phosphate glasses for which I have to study the decay time. I measured the decay time experimentally and also calculated the decay...

16 October 2020 951 7 View

What is the black block for Dark Current in a UV-Vis Spectrometer machine? How to insert it in a Spectrometer machine?

I am trying to construct a calibration curve on a new UV-Vis Spectrometer machine (T80+, PG Instruments Ltd). But when I start by setting the parameters using K-Factor method (pressing...

06 October 2020 2,521 3 View