At the sampling site in different habitats at various forest type like alpine grassland, tropical, subtropical forest. Being a beginner researcher, what should be intial scientific approaches to consider before collection of data in study site interm of highlighted key points such as pre and post sampling options, frames, techniques, heterogeneity, with respect to the way of consistently accuracy of research...
https://agsci.psu.edu/aasl/plant-analysis/plant-tissue-total-analysis/instructions-for-taking-samples-for-plant-analysis
I found this article very helpful. Good luck on your project.
Hurlbert SH. 1984. Pseudoreplication and the design of ecological field experiments. Ecological Monographs 54:187–211.
Sample using random quadrats across 5% of the area using at least 30 samples in at least three different areas, for replication. But remember, you can't record species or measure a variable if it's not in your quadrat! Aim for data that can be analysed via ANOVA or PERMANOVA and PCA :)
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