Contact Dr. James Ellis at

• Honey Bee Research and Extension Laboratory
• University of Florida, Institute of Food and Agricultural Sciences
• PO Box 110620, 1881 Natural Area Drive, Gainesville, FL 32611   USA
• Phone: 352-273-3969

READ the paper:

Fine JD, Shpigler HY, Ray AM, Beach NJ,

Sankey AL, Cash-Ahmed A, et al. (2018)

Quantifying the effects of pollen nutrition on honey

bee queen egg laying with a new laboratory

system. PLoS ONE 13(9): e0203444. https://doi.

org/10.1371/journal.pone.0203444

and try to contact the authors

Good luck

Itsic

Hello Risto Raimets ,

I recommend following the protocol of the OECD 239 test guideline for rearing honey bee workers in a laboratory setting. However, you need to adapt the feeding according to the requirements of honey bee queens (i.e. royal jelly only).

• http://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?cote=env/jm/mono(2016)34&doclanguage=en

The problem with RJ supply is that there are no quality standards. Ovber 90% of the RJ exports come from China, so you have a high risk to obtain contaminated material which would probably affect the outcome of your experiments. If possible, look for a local RJ supplier and order organic quality.

Good luck,

Richard

Thanks a lot!

following.