Hello, i'm isolating lactobacillus from commercial dairy products on MRS agar, i used serial dilutions but not satisfied with the final results.. it seems that the colonies are small as you can see in the picture .. is it normal ! how can i make it grow better?
- I used 9 ml of distilled water to prepare the dilutions with 1g of commercial yogurt samples
- I covered the plates with parafilm and plastic bags to create anaerobic conditions
- Scharlau MRS agar have been used
Lactic acid bacteria are Gram positive bacteria, rod or coccus shaped cell, negative catalase, non-motile and produce lactic acid as the final result of carbohydrate fermentation. Their colony size may vary from small to medium or large, same is the case with shape and pigmentation.
In my opinion the appropriate atmosphere should be used to provide ideal anaerobic conditions for growth, because I had this problem when I isolated Campylobacter jejune as it is a microaerophile, so I used its own atmosphere packs with an anaerobic jar because it is tightly covered but sometimes when something went wrong and I open the jar for a certain reason the colony growth on the next day is not satisfactory and they are small, so using its appropriate atmosphere with an anaerobic jar to get the satisfactory growth of the colonies may be appropriate solution... Best wishes in your work
Hi, many thanks because you addressed me, yes exactly the colony of Lactobacillus are small and sometime in a curved shape.
Lactobacilli are small, slender, nonmotile, gram-positive bacilli in chains. Immature colonies appear smooth, convex, and translucent. Organisms are microaerophilic or anaerobic and oxidase and catalase negative; they hydrolyze esculine and ferment carbohydrates.
Manal Hadi Kanaan Karima Galal Abdel hameed Alireza Shahab Lavasani Nivedita Sharma Thank you ALL so much for answering my question, that was helpful !!
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