Is there anyone who can suggest some protocols for FISH experiments with unicellular algae?

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Abhishek Mukherjee

Hello Dr. Carolina Chiellini,

There have been studies related to identification of diatoms similar to Skeletonema using FISH techniques. Although they are not unicellular but are microalgae nonetheless, the techniques may come in handy.

Regards,

Dr. ABhishek Mukherjee

Carolina Chiellini

Ok, thanks for the suggestion, I will try to find the papers!

Regards

Carolina

Giulio Petroni

Hi Carolina,

One of the problems is the overlapping of fluorescent signal from some of the probes used in FISH and auto-fluorescence of Chlorophyll and other pigments presents in algae.To my knowledge, only some fluorochrome are usable with algae