What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Sample is in a powdered form - how do I prepare for DLS?

I have this question from @Anurag Nath posted on an existing thread: https://www.researchgate.net/post/What_are_some_effective_sample_preparation_techniques_for_DLS_study To avoid confusing the...

06 January 2021 4,856 2 View

Does anyone have an insight into the behavioural response of fishes to moving wind turbine blades as seen through the air-water interface?

I am interested to find out if wind turbines located close to rivers or lakes, or perhaps even in marine locations, are a potential problem for fish. This seems to be a very under-researched area...

04 January 2021 3,468 2 View

Why is my TiO2 sol milky?

@Krunal Dholiya has sent me this question which I post below so he can get the benefit of many experts. Dear, I am preparing a thin film using TiO2 sol but i m fasing problem, with time it is...

23 November 2020 1,188 2 View

Zeta potential and concentration?

I have a question from @Pratima Adhikari that I will post on the open forum so all experts can contribute. Hi Alan, I hope you are fine and healthy. I have a question for you. I am currently...

22 October 2020 3,377 3 View

Which software can be used to prepare nexus file for phylogenetics analysis in Mr Bayes?

which software can be used to prepare nexus file for phylogenetics analysis in Mr Bayes? which format is best interleaved or sequential formats? Can you please explain?

22 February 2020 4,878 3 View

Possibility of inculcating INTELLIGENT MANAGEMENT SYSTEM to Higher education system?

The impact of IMS(INTELLIGENT MANAGEMENT SYSTEM) in the industry as well as economy in multiple countries at multilevels had been creating immense impact in the way the whole system was developed...

03 December 2019 4,284 3 View

What can be the possibility of inculcating behavioral finance to the existing studies conducted?

What are the possibilities of incorporating BEHAVIOURAL FINANCE to the existing stream of studies that already gave a clear base to the subject for Phd? What are the possibilities of looking at...

03 December 2019 5,884 11 View

What should be the basic text/document to refer upon for researches(especially social sciences)?

The fact that better knowledge on basic research comes out of understanding the existing techniques and methods is the key for a fruitful research. 1.Which should be considered as the primary...

23 November 2019 3,725 3 View

What causes the stability or instability of powders in suspension?

@Talat Zakeri has asked me this question by PM. In order that she can benefit from input from many experts I post it below: Hi, I have two powders from Sasol company. one of them is alumina and...

21 April 2019 8,410 7 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I separate Gertrud Pfister's profile and the profile of Gerald Gems?

we are co-authors of several publications. But I am the only and single author of most of my publications which are based on my own research. How can I separate the profiles?

21 February 2021 4,582 3 View

Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View

Change of First name?

In some publications you refer Deivid Chica (with Barranco) but the true first name is Maria Dolores Chica , in spanish Ma. Dolores Chica. Before I answer yes is an author Deivid Chica, but...

18 February 2021 5,004 3 View

How should I compute a new variable with z-scores?

I would like to create a new variable using 4 different measures: hIndex (range: 1-47), # of publications (range: 0-111), and 2 other measures (range: 1-7). I changed all of these variables into...

09 February 2021 4,866 3 View

Concentration of DNA?

Hello everyone! Is a normal value 295.8 ug/ul for cDNA? If not what does it mean? Where is the mistake? The measurement was done with nano drop. Thank you!

09 February 2021 6,202 1 View