Please what is the best solvent to dissolve heme?

I have tried NaOH and NH3 so far but can't dissolve well.Thanks for the assistance. I can be contact personally on this email:[email protected]

04 January 2021 5,528 2 View

PVDF membrane degradation?

Hi, I'm performing western blots on different samples and I'm facing a some problems after eletrotrasnfer with PVDF membranes. In general, during the incubations with the primary and secondary...

18 November 2020 6,412 7 View

What to do when you found a published paper that doesn't belong to you but has your name as the author?

I found a published paper online (attached here) that bears mine and colleague's names as authors but the paper doesn't belong to us. The work was claimed to be carried out in Kenya. We are in...

22 March 2020 4,860 7 View

Do you know a (validated) instrument for the assessment of state embarassment?

I've found quite a few scales for vicarious and personal embarassibility as a personality trait so far (i. e. Kelly & Jones, 1997; Maltby & Day, 2000; Uysal et al., 2014...). As I'd like...

24 February 2020 8,142 4 View

Are Peak Areas of different silylations comparable?

If I have an analyte, that has different silylations, like 2-TMS and 3-TMS, how can I compare the peak areas? Can I simply sum them up? What if theres a different ratio of 2-TMS/3-TMS in my...

03 December 2019 273 4 View

What is a good topic for Blockchain applications in IoT?

Hello everyone, at the moment I'm looking for a topic for my bachelor thesis. I was thinking about Blockchain and Smart Contracts for cyber-physical Systems, in order to use it for Smart Factory...

23 October 2019 3,694 5 View

How to carry out western blotting for a 233 kDa protein effectively?

Hi, I want to carry out western blotting for a 233 kDa protein. I was told to use a tris-acetate gel due to the size of the protein. However, I was wandering would I be able to detect the...

25 March 2019 6,308 4 View

How to set up Coupled Displacement Boundary Conditions in COMSOL?

Dear Community, I recently switched from ANSYS Mechanical to COMSOL Multiphysics. So far I was able to transfer my models to the new platform but I yet miss one feature of ANSYS in COMSOL. I hope...

29 January 2019 4,937 3 View

SpeedVac of DNA and salt concentrations: downstream effects?

I often use a speed vacuum concentrator to concentrate my DNA prior to restriction digests, DNA ligations, NGS library preparation or target captures. Usually, my DNA is eluted in 10 mM Tris-Cl...

13 November 2018 6,049 3 View

How do I calculate NDVI images with different thresholds using Sentinel-2 image?

I want to set different thresholds for calculating the NDVI images using Sentinel-2 for the purposes of fishing out river sediments in the Dry and Wet seasons. I am using the Semi-automatic...

05 November 2018 694 2 View

Do you agree with the just issued Great Barrington Declaration regarding the response to COVID-19?

The short and to-the-point text is here and I for one gladly co-signed this declaration, just as I also co-signed the balanced approach statement issued in July 2020 by 23 public health experts...

04 October 2020 8,795 11 View

What is the role of electoral politics in Hannah Arendt's conceptualization of the masses?

In Arendt, masses are distressed due to traumatic events, economic hardships imposed upon them, or political crises, systemically marginalized and pushed out of the boundaries of the political...

20 August 2020 5,099 7 View

How to extract polygons of species distribution from books?

If you need a distribution map from a book, how do people deal with that? Do u email authors to obtain the layers or is there a way to export the polygons from the book images? Colleagues and I...

20 June 2020 4,391 1 View

Does anyone use/have/try any antibody anti OVGP1 in oviduct cells/tissue?

I am looking for an antibody to show the expression of OVGP1 in porcine oviduct cells in culture. Is there one from Sigma and others form ABCAM. But an antibody is too expensive (at least for us,...

31 March 2020 7,273 2 View

Coronavirus and politics: what does the epidemic tell us about democracy and other regimes?

Most of us experience far-reaching restrictions on private and public life, introduced by governments all over the world because of the coronavirus epidemic. Generally, these restrictions make a...

22 March 2020 6,992 95 View

Can anybody link me to current research rgarding the implications of the Venezuela crisis?

I am currently looking for up to the minute research or research within the last 18 months or so which details the effect that the venezuelan crisis is having on venezuelan society. Preferably...

31 December 2019 2,179 3 View

Are the richest countries rich because they inverts a lot in science? or they inverts a lot in science because they are rich?

When will South America (or specifically Argentina) win a science Nobel Prize? Another year without a Nobel Prize coming to South America The last time that a South American scientist was awarded...

09 October 2019 430 3 View

Do anarchist principles make the work of science possible?

07 August 2019 286 1 View

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07 August 2019 6,051 3 View

Is there volcanic fumarole manifestations within the Argentinian Altiplano-Puna Volcanic Complex at Centrral Andes? Which volcanoes do show this?

I'm searching for information (papers, reviews, conference abstracts) about volcanic fumaroles within the Altiplano-Puna Volcanic Complex, at Argentina. I'm looking for ages and composition of...

31 December 2018 238 2 View