IS MALDI biotyping alone sufficient for identifying pneumococci after isolation?

06 May 2022 1 8K Report

Is it necessary to go through the conventional Gram stain, optochin, and bile solubility test after isolating my pneumococci on selective media if my intention is to identify the isolates by MALDI-TOF MS? The sensitivity from the couple of studies I read is quite high.

Newton Verbisck

Hi Reuben,

Yes, you can identify S. pneumoniae using MALDI-TOF biotyping, for instance, with MALDI Biotyper from Bruker Daltonics.

You may have a look to the following:

Article Improved Differentiation of Streptococcus pneumoniae and Oth...

Article Utility of MALDI-TOF MS as a new tool for Streptococcus pneu...

You may go through conventional microbiological methods for characterization of your isolates in paralel, but identification by MALDI-TOF MS alone is reliable.

Hope this helps, good luck.

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