Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Why am I getting 2 bands for Gibson Cloning Vector PCR?

Hi, I designed Gibson primers (2 primers for fragment and 2 primers for vector) using snapgene. Then I ran PCR separately for Fragment and Vector. Fragment DNA was found to be the correct sized...

11 January 2021 9,500 2 View

Was there any pesticide disasters other than Bhopal and Kasaragood tragedy around the globe?

Pesticides become prime compo in agricultural activities to meet the growing demand of the human population. But indiscriminate usage and mishandling led to two disasters in India such as Bhopal...

11 January 2021 3,790 7 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

Can we mix elemental powders of 325 mesh using an agate mortar and pestle?

Elemental Powders like Titanium, vanadium, tungsten, zirconium, hafnium.

04 January 2021 7,357 1 View

How can we say a species of insects got extinct?

02 January 2021 4,232 10 View

No title

It is important for me to know the opinion from your point of view as researchers.

30 December 2020 988 2 View

How can i calculate diffusion barrier of Li ion on graphene nanoribon using SIESTA?

I'm a new siesta learner, I'm working on lithium-ion batteries, I have calculated adsorption energy but i am not able to calculate diffusion barrier of Li ion. kindly help me how to calculate...

14 January 2021 2,688 1 View

How to calculate Basis Set Superposition Error (BSSE) for interaction energy in SIESTA 4.0.1 code?

Hello, I'm calculating the interaction energy between molecule and graphene sheet using Eq. (E_i = (E_graphene + E_molecule) - (E_graphene+molecule). I have employed a numerical atomic orbital...

10 November 2020 8,467 2 View

How nanoclusters differs in their selectivity towards adsorption based on their shapes and sizes?

I want to perform adsorption using DFT code but I can only create nanosheet like structure - supercells from primitive and use them to check their adsorbing selectivity towards certain gas. I...

06 June 2020 1,643 5 View

Can we consider optimised Transnational vectors for bost calculation in SIESTA ?????

When we interact any 2D sheet with small molecule (eg. H2O, O2 ), Transnational vectors of whole system changes after optimisation. So can we consider for post calculation like PDOS plot, charge...

08 March 2020 3,066 2 View

How to calculate the current-voltage relationship by Transiesta module in SIESTA-4.1-b4 version?

Hi, I am new in SIESTA and using version 4.1-b4 for the transiesta calculation. But the following the tutorial videos, manual and examples the calculations are not getting completed properly...

15 January 2020 7,074 3 View

Incorrect bandstructure of zigzag ZnO ?

I'm a beginner in siesta code. I'm trying to simulate zigzag Zno structure using siesta. After optimization I found that Zn atoms form an unwanted bond which I think could be reason for incorrect...

03 January 2020 7,833 3 View

How to calculate the current-voltage relationship by Transiesta module in SIESTA-4.1-b4 version?

Hi, I am new in SIESTA and using version 4.1-b4 for the transiesta calculation. But the following the tutorial videos, manual and examples the calculations are not getting completed properly...

31 December 2019 8,109 3 View

How can we analyse magnetic property from SIESTA output file ?

What is significance of following parameters in *.out file after SIESTA run ???? (Free)E+ p_basis*V_orbitals = -6655.206619 (Free)Eharris+ p_basis*V_orbitals = -6655.206622 spin...

04 December 2019 7,481 1 View

How can I calculate DOS for g-C3N4 more accurately?

I am working on g-C3N4 by SIESTA code. I optimized structure, E cut off and Monkhorst Pack(Na=3, Nb=3, Nc=1). I optimized Monkhorst Pack by single point calculations. I plotted band structure of...

10 November 2019 6,762 1 View

Regarding Band gap calculation in siesta ?

Heloo All, I want to study the electronic properties of double wall carbon nanotubes using SIESTA code. For that purpose i calculate the band gap. My band gap value differs from the literature....

10 November 2019 300 3 View