thanks for sharing ideas

I believe i have to redo the procedure to provide a strong validity.

Respected Researchers,

I agree with Dr. Bhoj R. Singh and Hero M. Ismael.

I would like to add an additional dimension to the ongoing discussion, i.e., Concentration dependent synergy.

We all know that herbal extracts are composed of multiple phyto-constituents which might act synergistically or antagonistically at different concentrations. So, such concentration dependent synergism or antagonism might be one of the probable reasons for such observations. 

Regards

Pallavi Thakur

Researcher

Institute of Nuclear Medicine and Allied Sciences

DRDO, Ministry of Defence

Is there any paper available that support the idea that at lower concentration - the effect is greater than higher concentration. 

thank you sir

Are your test compounds precipitating out at higher concentrations?

yea its true for some essential oils. Actually i am using Tween 20 for dissolving oil in water. I was thinking - is that due to precipitate that i am getting my results in such manner.?

 yes there is possibility, it may be active at low concentration with available high nutrients  and not in higher concentration  with low nutrients.