Which is the official website to get the solar radiation data?

Please let me know

05 January 2021 6,370 5 View

How good is SYSTAT software vis-a-vis SPSS?

Anyone who has used both SPSS and SYSTAT software and can provide insight as to whether latter is as good? Are there any hitches in SYSTAT? Planning to shift to SYSTAT, so seeking information....

17 March 2019 8,021 2 View

How saturation magnetization and coercivity varies with nanoparticles size and temperature?

explain in detail

03 June 2018 9,795 4 View

What should be the power of laser that it can penitrate through 1cm thick quartz surface?

For illumination of green light on the sample through 1 layer of approximate 1cm thick quartz surafce, what should be the power of laser source?

19 June 2017 7,321 2 View

In MD simulation of polymers, how many chains of polymers must be taken in order to get correct results (one chain has 200 monomers)?

The monomer has 9 atoms each.

26 April 2017 2,605 3 View

Problem in DNA extraction?

Hi, How i can remove polysaccharides and polyphenols during the DNA extraction from Terminalia arjuna, i have tried various methods but unable to find DNA in purified form? I have tried many...

17 January 2017 7,810 2 View

How to find lattice mismatch in % from the lattice constant of two materials?

How to calculate lattice mismatch theoretically?

14 January 2017 8,062 22 View

How i can extract DNA in purified form from the leaf samples of Terminalia arjuna leaves ?

31 July 2016 8,559 3 View

What if content of gc is low in gene of interest while designing primer in pcr ?

17 February 2016 6,056 3 View

How much temperature will be suitable to dry leaf samples for preservation without damaging DNA?

15 December 2014 5,773 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View