The medium gets precipitated when all the salts were added .Kindly suggest a good technique.
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A mineral medium for studying the growth of Nitrosomonas europaea was developed and examined. The
medium was defined in terms of chemical speciation by using chemical equilibrium computer models. The
medium significantly increased the metabolic activity of the organisms compared with previously developed
media, yielding a specific growth rate as high as 3.0 day-' (generation time, 5.5 h). The specific growth rate
was enhanced by increasing the inoculum and was linearly correlated with the inoculum-to-total-culture
volume ratio on a semilog scale. A reproducible growth rate for N. europaea was obtained with this medium
under controlled experimental conditions.
Q. The medium gets precipitated when all the salts were added .Kindly suggest a good technique.
Ans. The medium components without salts and all the salt solution are separately autoclave and it should be mixed at the time of inoculation, to avoid its precipitation.
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