Luteolin is a flavone belongs to flavonoids family. Luteolin was found in some leafy green vegetables such as spinach and watercress.
In the field of olive oil luteolin content could be checked by rp-hplc analysis. In general there is a previous extraction phase of polyphenols (for example by a mixture of methanol/water) and then the methanolic phase, which contains the polyphenols, is injected into the hplc system. If your detector is not a destructive one (and i think that in general it isn't a destructive one in hplc), you can try to collect luteolin after elution. You could check on web for polyphenols extraction protocols from olives/olive oil as an example.
Thank you Dr Giacomo for the answer. I understand what you say about extraction of luteolin or generally flavonids based Hertog 1993 and Abdel-Moemin 2004 methods. But i need a clear procedure to isolate luteolin and a test to confirm the presence of it. Thank you again for participation
you need to study, physical and chemical properties to extract , at first, flavonoids , then prepare specific column to bind luteolin (prepare solvent system)
I think you must prepare mix solvent to gain luteolin
Thank you Dr Eshak for your help. I will be very pleased if you have a paper to attach. Thank you again for participation.
Hi,
Try to extract Luteolin from the crushed leaves using methanol-water mixtures (you may use different organic solvent with different polarity), then you can identify and isolate using RP-HPLC.
The paper in this link might be useful, Good luck
http://www.sciencedirect.com/science/article/pii/S1995764514602480
Dear Dr Djalma,
Thank you for your answer, however could you please explain more. Best regards
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