I collected various samples from the coastal region, which will be the best media to culture the microalgae from that sample under a laboratory scale.
Dear Sujit Kumar Nayak
Please follow the articles
https://www.sciencedirect.com/science/article/abs/pii/S2352485522000159
https://www.mdpi.com/1996-1073/13/4/898/htm
This would be helpful for you
All the best
Thank you
Chandan
Dear Sujit,
Patiently, is probably the best way! Unless you have a cell sorter. You will need to continuously carry out single cell isolations in a well plate moving the cell of interest into clean, appropriate media under species specific optimal conditions until that cell divides to become the dominant strain. At that point you may be able to carry over more than one cell into a fresh well. Then, it may be necessary to treat the new culture with antibiotic or anifungal agents. Sadly, it will not always be possible, as many species will not tolerate the conditions of single cell culture. Best of luck with it!
it's depending on species isoleted ( dinflagellates; diatoms or others ) , you ca use L1 or F2 media.........
conditions for growing changes either ( T°, salinity, concentration of nutrients, irradiance....)..
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