I prepared flower buds to karyotype for Yam crop. Flower buds were collected at 10am and directly fixed 3:1; ethanol and acetic acid for 24 hrs. and kept at room temperature. Pollens were removed from anther, squashed and stained with FLP ORCEIN solution for 60 to 90 minutes to allow enough dye accumulation. The prepared samples, mounted between slides and coverslip, were observed under 40 X magnification and captured at 100X magnification with oil immersion using an optical microscope. Another steps involves squashing of flower buds without removing anther/pollen because of using small buds size at tip location. I am only observing mitotic cell division. Please, download the chromosome pictures here that someone can guide me further:
You have to look for haploid cells. See:
https://www.yourgenome.org/facts/mitosis-versus-meiosis#:~:text=Cells%20divide%20and%20reproduce%20in,two%20types%20of%20cell%20division.
Hello Andrew, thank you very much. The somatic cell of this specie (Diocorea rotundata) is 2n=2X=40. Does that mean I should look for chromosome of 20 in the cell metaphase?
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