Dear Friend,
It is well-known that when there is a single emission peak in the PL spectrum, the maximum emission wavelength corresponds to the wavelength of that peak. However, when multiple emission peaks are present, how should the maximum emission wavelength be defined? Should it correspond to the peak with the longest wavelength or the one with the highest emission intensity? Are there any references or literature that support this definition? Additionally, how should this be addressed in the context of absorption spectra?
I look forward to your insights on this matter.
Sincerely
Robin King
The maximum emission peak typically refers to the wavelength with the strongest fluorescence response. If there is significant absorption in the spectral region where the unattenuated emission maximum would appear (e.g., at high analyte concentrations), a secondary inner filter effect may cause an apparent shift in the emission maximum, so that should be kept in mind, as the maximums can change.
To clarify the value of the peak maximum, you should decompose the spectrum into components, i.e. determine the fitting curve. This is done by Gaussian functions in the work https://www.researchgate.net/publication/341888166_A_Site-Specific_Self-Assembled_Light-up_Rotor_Probe_for_Selective_Recognition_and_Stabilization_of_c-MYC_G-Quadruplex_DNA
This procedure can be done using the free Fityk program https://fityk.nieto.pl/
Before the fitting curve, it is necessary to convert the x scale from nm to eV.
Mikhail Zimin Dr. Mikhail Zimin, Thanks for your answer. As presented in the Figure, two absorption peaks can be observed. So,which peak should be the maximum absorption peak? 334 nm or 445 nm ?
Robin King I don’t understand why the maximum absorption peak is needed, but based on the definition, this is the peak with the highest optical density (or intensity in the case of luminescence and maximum emission peak).
On example of the article, these are peaks at 334 and 317 nm.
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