Hey everyone. I need help with the following. I need to assess the yield of extraction of oil from microalgae, and one of my parameters is the biomass:solvent ratio. My tutor told me to do it in the following ratio; 1:15. But from my calculation its kinda weird, because I got 0,05g of biomass and 0,470uL for solvent. I read some papers and the ratios described are from 1:100 and more, so I don't know if I'm calcuting wrong the ratios or are they ok.
Thanks in advance
Hello, Paula. Are you working in weight/weight or in weight/volume when applying ratios? Because if you are working directly with weight/volume ratio, then your calculation is not correct. With a 1:15 biomass:solvent ratio in terms of weight/volume, you should use 15 parts solvent for every 1 part biomass, so if you have 0.05 g of biomass, you should use 0.75mL (or 750 µL) of solvent. If you are working with weight/weight ratios instead, then I'll need to know the density of the solvent to calculate the correct ratio. Hope that helps!
Thank you José Ramón Martínez Guillén . Yes, i'm working in w/v ratio, so I'm gonna check my calculations to see where I get the mistake.
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