Hi everyone,
I just checked sigma website and found Trypan Blue powder got two different versions: 40% and 60% in terms of dye content. When I googled, usually what people say is we just need to weigh 4mg powder and dissolve into 1ml PBS buffer to constitute 0.4% TB solution. I am quite confused as if we have these two different version of TB powder, do we need to take into account the percentage of the dye content within the TB powder? If so, what shall I do to get 0.4% in both case?
Hi Zhuoran, if you dissolve your material (40% dye content) in buffer at concentration 10 mg/mL, you will obtain desired 0.4% TB solution. As you can see, it is very simple.
Hi Maksim,
Thanks for your input! That makes me know that the online protocol may be wrong about the preparation as they did not take into account the dye content. I will refer to your suggestion!
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