It is not desirable to be more than 0.1-0.2% and you should also expose one group of cells or animals with the same amount of DMSO.
Dear Khan,
It depends on how much DMSO dissolves your extract content. You should use try and error method. Also, treat your vehicle control with the same amount of DMSO to neutralise the effect of DMSO among your groups. Although less possible, if it is not soluble enough, you may have to use another solvent. Also, your study type is important.
Its depends on the solubility of extract in DMSO. But when you will add the extract in media for anti-cancer activity study, the final DMSO concentration not more than 0.1%. The DMSO create toxicity to cells above the concentration of 0.1% (But it also depends on the cell type).
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