How can I design Primer for PCR of a specific gene perfectly?

I want analyze specific polymorphism of a specific gene with Restriction Fragment Length Polymorphism (RFLP) method. So How can I design primer perfectly? Pls tell me the perfect procedure..

06 July 2015 6,443 4 View

How can I design perfect primer for specific gene with perfect PCR condition?

Gene, PCR, RFLP, Genetics, Pharmacogenetics, Pharmacogenomic

04 May 2015 2,734 3 View

How many pathways are responsible for graft rejection in human organ transplantation?

Stem Cell, Molecular Biology, Organ Transplantation

03 April 2015 3,438 10 View

What is the standard procedure of designing the primer for PCR of specific gene?

I want to synthesize few gene from the previously extracted DNA from leukocyte.

07 August 2014 7,509 0 View

What is the future of biogas in Asia?

What is the future of biogas in Asia?

31 December 2020 8,821 16 View

What country in Asia produces the most renewable energy?

What country in Asia produces the most renewable energy?

25 December 2020 8,762 24 View

Please name companies in Asia or India who carry out whole human genome sequencing with high quality for less cost?

22 December 2020 4,495 3 View

Most of the plastic is shipped to Asia?

Dear Sir I came across your paper. First of all, congratulations on your amazing research article. Loved it! Also, I am very interested to know, why is the waste that is collected in the...

14 December 2020 6,342 4 View

Does anyone know of any South American or African frogs with ultrasonic components in their calls?

There are a few examples of Asian frogs (Odorrana and Huia) with advertisement calls that contain either audible and ultrasonic components, or purely ultrasonic components, I was wondering if...

26 October 2020 2,906 16 View

Is there any open datasource to study sea level change in parts of Asia?How to predict the rate of SLR for coming 50 years ahead?

I am looking for datasets to study sea level change in parts of Asia since i do not have any field observation. I searched in NASA Sea level portal but it left me confused how to process those...

09 October 2020 9,795 1 View

Is there any open datasource to study sea level change in parts of Asia?How to predict the rate of SLR for coming 50 years ahead?

I am looking for datasets to study sea level change in parts of Asia since i do not have any field observation. I searched in NASA Sea level portal but it left me confused how to process those...

08 October 2020 3,152 1 View

Literature on traditional/ancient slaughtering patterns of goats/sheep?

Any recommendations? I'm mostly (but not exlusively) interested in eastern Mediterranean and West Asia...

09 September 2020 7,344 3 View

Any Primary data studies on mainstreaming online higher education in Asia ?

I would like to have information on current studies on challenges and opportunities for mainstreaming online education in Indian, Malaysian , Indonesial or Sri Lankan Universities

25 August 2020 5,180 3 View

What could be the practical mechanism to resolve water dispute between India and Pakistan?

As water is crucial element for all human beings on earth. Due to climate change, water is going to be scarce element on earth. India and Pakistan are two rival states in Asia but on the other...

25 August 2020 8,444 5 View