How long can dissociated cells be kept on ice before performing scRNAseq?

01 January 2019 1 8K Report

We would like to perform dropseq but the scRNAseq instrument is located off-site. Would it be best to perform the single-cell dissociation where the machine is located or can they be transported to the machine that is off-site? Once cells are dissociated, do they need to be used in the scRNAseq instrument straight away?

Any advice would be much appreciated! Thanks!

Benedikt Bauer

I haven´t read the whole paper but maybe this is something for you:

Article The analysis of viability for mammalian cells treated at dif...

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