Hello,
I am currently employing advanced Marfey's analysis on a small peptide in order to determine absolute configuration of each amino acid. However, I'm having an issue in which the tyrosine residue appears to be degrading or modifying during the hydrolysis (6N HCl, 110 C, 24 hrs).
Does anyone have suggestions on how to prevent this from happening? I've read that adding phenol to the hydrolysis might help but not sure if that works.
Thanks,
Peter
Ok, great. Appreciate the feedback! I'll update this message chain when I have the results.
Any other feedback regarding the question would always be welcome.
Just wanted to give an update --
I added 0.1% (w/v) of phenol to the hydrolysis solution and was able to prevent at least a partial population of tyrosine from reacting with the trace radicals. This was exactly what I was looking for. Appreciate the help.
Another note -- I could have probably added less given that it appeared phenol was not completely soluble at 0.1%.
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