During ration formulation of dairy animals lysine, methionine, and other amino acids do not consider how to meet their requirement?
They obtain it from metaboizable protein ( MP) which is partially obtained from non-degradable dietary protein and microbial protein.
Use of non- protein nitrogen to make microbial amino acids, digestion of rumen undegradable proteins in abomasum and breakdown of rumen degradable protein by microbial in rumen thus they proliferate and move to abomasum and act as protein source
As the colleagues above mention. The first chamber of the stomach (rumen), produce and process the feed given to ruminants. Part of the protein on the diet will remain intact, but mostly the ruminal fermentation of feed by the symbiotic miroorganism (MO) in the rumen provides most of the required aminoacids(aa). The intact protein and the ruminal microorganisms are digested in the true stomach (abomasum) and aa are absorbed in the lower part of the gastrointestinal tract.
Some researchers argue for the need to include aa in the diet, especially on high productive cattle, because some essential aa, like the ones that you mention, can be limiting factors. In my opinion, and undoubtedly, the aim of every balance diet will be to maximise the protein produced by ruminal MO, as it provides the optimal range of aa for ruminants (i.e. aa profile in MO = aa profile in Milk).
Part of it is supplied through feed and concentrate compounds and part through microbial synthesis by rumen microbes.
Please have a look at the Protein chapter of the new (2021) NRC 'Nutrient Requirements of Dairy Cows, Eighth revised edition'
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