Problems with library virus generation?

Dear all I cloned an sgRNA library into a pLenti plasmid backbone (puromycin selection marker) by golden gate ligation and transformation. However I cannot produce any virus from the library...

25 October 2020 3,820 2 View

How can I create a Venn diagram using SPSS?

I have 5 sets with 200 variables.

14 October 2020 9,681 4 View

How to model water acidification using CO2 and acids ?

Hello, I am trying to model the amount of CO2 vs mineral acid (HCl, H2SO4) needed to acidify water with various alkalinity conditions. It would be helpful if I can get some suggestions on the...

04 May 2020 9,798 8 View

Transfection of HEK293T cells with linear dsDNA?

Does anyone have experience transfecting or electroporating linear dsDNA into HEK cells? If yes what is the size limit, what size have you tried and worked. In principle, I want to do HDR, and...

25 March 2020 5,649 3 View

How the Aluminium Oxide layer on Battery Terminals affect the performance?

Hello Everyone, I am currently optimizing the production process for the battery packs. I would like to know how the great the increase in contact resistance with an aluminium oxide layer on the...

21 May 2019 2,782 3 View

Why DCIR value changes in the Battery?

I am using LiFePO4 battery, I measured DCIR using the discharge pulse. I found that the measured value of DCIR changes with respect to both pulse current and SOC. I provided adequate rest period...

28 January 2019 5,227 4 View

How to measure the energy consumption by DC system?

I am designing Battery pack for a DC system. In order to find out the capacity required for the system, I need to calculate the energy requirement (Variable load, Idle condition). Since the...

10 September 2018 9,766 8 View

How to test BMS?

I would like to know how a BMS from a different Manufacturer can be tested? I want to make sure whether the BMS works as per our requirement and also ensure its operation as per the data...

08 May 2018 5,204 3 View

What is Battery Size Factor? when it should be used?

Hello Everyone, I would like to know what is Battery Size Factor? How it is related to Battery Testing and when it should be considered? Thanks in advance

29 April 2018 7,871 4 View

How to find out diffusion coefficient using warburg impedance?

11 September 2017 1,196 13 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

How to prevent ANFIS Model from negative prediction values in MATLAB?

I have input and output data set for "ANFIS modeling in MATLAB", and I am getting some negative predicted values of output in testing. However, the predicted values of output in training are...

28 February 2021 3,459 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

D2D implementation in Matlab in Vienna LTE software?

Hi Hope you are well. Can you please share your code for D2D implementation in Matlab. I want to implement D2D in Matlab based Vienna simulator and struggling to deploy D2D. Thanks

24 February 2021 9,378 3 View

Multi effect distillation modeling in EES?

Dear colleagues If anyone has a model of multi-effect distillation in EES or MATLAB software, please send it to me.

24 February 2021 7,282 1 View