I'm now purifying a modified tripeptide which is quiet non-polar and sensitive to acidic condition. Thus I added triethylamine (TEA) to neutralize the HPLC fraction which contains 0.1% TFA and lyophilize them. But I could not get rid of the residual liquid by lyophilization, I believe it would be the TEA-TFA salt. I was thinking to use a SEC C18 (like waters SEP-PAK C18) column to desalt my sample. If there is any other practical way for me to remove TEA-TFA salt from my sample?
Thank you for your kind help.
You can check this RG link
To remove traces of TFA you can use exsiccator with KOH and - optionally - some heat. If you have the salt with TFA you could dissolve your product in water add some NH3 - until you have slight alkalline conditions - and extract your product with CHCl3 or DCM, evaporate and dry over KOH.
https://www.researchgate.net/post/How_can_I_remove_TFA_in_a_product
How to remove TFA salt
https://www.researchgate.net/post/How_to_remove_TFA_salt
Peptide TFA removal service I Smart-Bioscience
Anionic TFA counterions interact with the free amine group at the ... The removal of TF- Acetate salts bound to the peptide is a challenging ...
https://www.sb-peptide.com/peptide-service/available-options/peptide-tfa-removal-service/
- Badges
- Science topic
- Similar topics
- Chemistry
- Sulfites
- Inorganic Chemistry
- Inorganic Chemicals
- Salts