How can I get Chromosomal location of fungal genes?

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Why I amGetting non specific bands for my MBP fusion protein after GPC?

Hello Every1!!! I am trying to purify my MBP fused protein by affinity chromatography using amylose resins but getting to many non specific bands.. therefore i tried gel filtration but again and...

07 August 2019 2,473 4 View

Double digestion problem ?

In order to do a cloning I am trying to do double digestion of my vector with Sph1 and Not1 enzymes , the sites are 14 bases apart. My question is - is it appropriate to do double digestion at a...

03 April 2019 647 3 View

How much concentration of template(Rec Plasmid) required to see a fallout of 166bp?

Hi all, I m very much confused for a double digestion reaction to check a fallout of 166 bp which has been cloned in 6900bp vector. I have took around 1500 ng of my recombinant plasmid(6900+166...

03 April 2019 7,114 4 View

Any suggestion why i am getting higher protein band size??

Hello all! I am doing SDS-PAGE for my protein of predicted size of 53.5kda bt unexpectedly I am getting bands of around 60kda.. I have done this 3-4 times and Everytime the required band is about...

01 February 2019 5,632 8 View

Does Mutation In Ribosomal Binding Site cause any interference in protein Expression?

Does single nucleotide mutation in Ribosomal Binding Site of an expression vector hamper protein expression? As there is no band could be seen in SDS-PAGE..

07 August 2018 4,152 5 View

My clone ( from cDNA) after sequencing reveals 2bp deletion in , should I use SDM for repair?

My clone ( cDNA Clone) has 2 bp (C&A) deletion as a result their is frame shift and no protein expression is taking place. gDNA clone reveals no deletion but all my cDNA clone reveals 2bp...

06 July 2018 10,071 9 View

I m trying to clone one gene from cDNA(502bp) and gDNA(600bp), during PCR i m getting amplification from gDNA only not from cDNA, any solution??

Same cDNA is used for other amplifications and i m getting result for that ,, actually the Tm of the primers is too low unfortunately it is around 35 degree , when i put an PCR at Tm 28 i got some...

01 February 2018 6,781 6 View

Is this website real?

Is this website real? https://isar.org.in/event/registration.php?id=2434532

08 August 2024 484 1 View

U you think We need a website software of Blackbody radiation law expert software?

A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index

07 August 2024 1,706 0 View

Gromacs first step of minimization ?

I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...

07 August 2024 2,598 1 View

Is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license?

Dear friends, does anybody know that is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license? Thanks.

05 August 2024 7,098 3 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How to find projects for a team of professional developers in AI?

We are looking for projects for a team of professional developers About us We are a team of experienced developers with over 10 years of experience in various programming fields. Our mission is to...

30 July 2024 8,377 0 View

How to change the version of the article full-text pdf file?

How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...

30 July 2024 3,229 2 View

How to get article and literature review templates (for publishing) from websites like Elsevier?

Hello, I want to know how to get the templates from the Elsevier official website (their last updated if exists)? I would like to know the steps if possible.

26 July 2024 3,264 2 View

Are there any alternatives to PhenoScanner?

I am currently conducting a mendelian randomization study, and I was attempting to use PhenoScanner to look for potential confounders associated with the selected SNPs (any SNPs significantly...

23 July 2024 5,703 0 View