How can I get Chromosomal location of fungal genes?

More Ravi Shankar Gautam's questions See All

Why I amGetting non specific bands for my MBP fusion protein after GPC?

Hello Every1!!! I am trying to purify my MBP fused protein by affinity chromatography using amylose resins but getting to many non specific bands.. therefore i tried gel filtration but again and...

07 August 2019 2,373 4 View

Double digestion problem ?

In order to do a cloning I am trying to do double digestion of my vector with Sph1 and Not1 enzymes , the sites are 14 bases apart. My question is - is it appropriate to do double digestion at a...

03 April 2019 562 3 View

How much concentration of template(Rec Plasmid) required to see a fallout of 166bp?

Hi all, I m very much confused for a double digestion reaction to check a fallout of 166 bp which has been cloned in 6900bp vector. I have took around 1500 ng of my recombinant plasmid(6900+166...

03 April 2019 6,999 4 View

Any suggestion why i am getting higher protein band size??

Hello all! I am doing SDS-PAGE for my protein of predicted size of 53.5kda bt unexpectedly I am getting bands of around 60kda.. I have done this 3-4 times and Everytime the required band is about...

01 February 2019 5,566 8 View

Does Mutation In Ribosomal Binding Site cause any interference in protein Expression?

Does single nucleotide mutation in Ribosomal Binding Site of an expression vector hamper protein expression? As there is no band could be seen in SDS-PAGE..

07 August 2018 4,068 5 View

My clone ( from cDNA) after sequencing reveals 2bp deletion in , should I use SDM for repair?

My clone ( cDNA Clone) has 2 bp (C&A) deletion as a result their is frame shift and no protein expression is taking place. gDNA clone reveals no deletion but all my cDNA clone reveals 2bp...

06 July 2018 10,000 9 View

I m trying to clone one gene from cDNA(502bp) and gDNA(600bp), during PCR i m getting amplification from gDNA only not from cDNA, any solution??

Same cDNA is used for other amplifications and i m getting result for that ,, actually the Tm of the primers is too low unfortunately it is around 35 degree , when i put an PCR at Tm 28 i got some...

01 February 2018 6,688 6 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View