What are the probable reasons that in my LCMSMS chromatogram, the background noise is absent?

I am trying to learn LCMSMS. For the purpose mentioned here, I am trying to make a standard curve of erythromycin. The solvent system is 1:1 water and acetonitrile. I got pretty good r-squared...

08 August 2020 9,768 3 View

Number of cores and nodes on running vasp on HPC?

I wanted to know how do you determine how many cores and noes you will need to perform an DFT analysis for particular molecules?? I normally use two cores for all my calculations and it pretty...

30 July 2020 4,279 5 View

How the INCAR file is different for SCAN and PBE+U for VASP?

Hi, I am trying to run a SCAN functional calculation in vasp. I have run the DFT for GGA+U in VASP before. So I was wondering what I need to deduct and add to my GGA+ U INCAR file to prepare it...

11 July 2020 334 3 View

Microbial protein changing its color due to stress why?

Microbial protein changing its color due to stress factor after two days of incubation .....

23 February 2019 1,919 2 View

What is the advantage of having trilobe and quadralobe cross-section of catalyst extrudes in gas phase high pressure reaction?

In fixed bed catalytic reaction, the shape of catalyst has plenty role to control the kinetics of the reaction. People have used trilobe and quadralobe cross section of catalyst in preference to...

24 October 2018 1,541 7 View

How can I change the pH of zinc acetate solution ?

I want to change the pH of zinc acetate solution. But when I add NaOH, a white precipitate was formed.

17 March 2018 4,294 1 View

What type of mice is appropriate for cancer study ?

I want to study the effect of radiation on malignant tumor cell and also evaluate the stress during treatment in mice model

07 November 2017 8,243 2 View

What is the best method to prepare cell lysate from mice skin epidermal tissue?

06 February 2017 2,474 4 View

Does anyone knows, how to calculate overpotentials from electrochemical data. i.e ORR/OER polarization curve or any other else??

I want to calculate overpotential from electrochemical data, for that purpose i required appropriate method to calculate same one.

08 February 2016 3,627 11 View

How to extract and purify membrane proteins expressed in Mammalian cells HEK 293 ?

I need some advise in purifying a membrane protein which is expressed in mammalian system HEK293.

19 May 2015 9,695 4 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View

How to build fluid structure interaction simulation for elastic ring squeeze film damper in COMSOL?

How to build fluid structure interaction simulation for elastic ring squeeze film damper in COMSOL?

22 February 2021 5,408 1 View

How to generate AA6061 alloy or any aluminium alloy crystal in material studio?

I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.

22 February 2021 1,410 1 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

How to do a surface treatment of uncoated TiO2 with polymer?

Basically, I would like to know how to treat or coat on the uncoated bare TiO2 (Ti-OH rich) surface with polymer which has anchoring groups in the polymer structure like trimethoxygroups. What is...

21 February 2021 4,657 6 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,633 1 View