05 February 2025 0 6K Report

Hello!

I am interested in extracting RNA from fern gametophytes, which are very small. I am curious if anyone has used the RNAqueous kit for plant tissues and, if so, how well it worked?

More Mari Wilson's questions See All
Can anyone kindly suggest research topics in the area of thermodynamics, heat transfer, fluid mechanics or renewable energy?

I am an undergraduate mechanical engineering student seeking suggestions on topics related to either thermodynamics, fluid mechanics, heat transfer or renewable energy for my final year project....

24 July 2024 4,058 5 View

Collecting/storing plant stable isotope samples?

Hello! I am planning to collect plant leaf samples for stable isotope analysis. I will be collecting them from the field and storing fresh tissue on ice. I have heard that bringing along a food...

01 July 2024 9,125 1 View

¿Qué estrategias en TIC son efectivas para apoyar la implementación exitosa del ABP en una escuela multigrado?

antecedentes de investigaciones relacionadas con la pregunta y el restreo bibliografico que soporte esta pregunta.

01 May 2024 4,237 0 View

Are you interested in therapeutic benefits of systemic nitric oxide in human disease?

We have developed an orally delivered compound that elevates serum nitric oxide where the NO has a circulating half-life of ~5 hours.

14 April 2024 6,554 2 View

Optical applications of DNA folding in composite functionalized nanoparticles?

Well, my boss instructed me to study this topic, I am using cadnano software, but I have no idea of how to generate a real DNA helix (maybe a box or a smiling face), and as far as I'm concerned,...

08 April 2024 7,949 0 View

How do I add authors (that are not in the list provided) to a new publication?

Hello, I want to upload an article just published, but the list of authors provided does not include my co-authors. How do I add my co-authors when the area of inquiry does not let me?...

03 April 2024 3,372 2 View

What are the absolutely essential "must read" books or articles on Christian Esotericism and Hermeticism?

I am interested on the application of Christian esotericism and Hermeticism to the development Of Orthodox Theology and spirituality (Sophiology, Theosis, theological anthropology). I am also...

25 March 2024 2,449 6 View

Seeking US-based reviewer in IT, Healthcare Management/Public Health, for book proposal review? Expertise and insights needed?

The purpose is to find an external reviewer for a book proposal, which has been submitted to a US publisher.

09 March 2024 7,593 9 View

What is a Healthcare Management Service Organization (MSO)?

What are the advantages offered by MSOs that fuel their demand in the healthcare sector

20 February 2024 5,956 5 View

What is responsible for the corrosion of steel rebar members in concrete pillars as they do not come in direct contact with oxygen and water?

Steel rebar members used in reinforced concrete pillars. They do not come directly in contact with Oxygen and water so why do they corrode.

23 January 2024 4,702 6 View

Similar questions and discussions
Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

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RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

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How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

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