I am working with hem protein. I built toplogy files for hem and ligand using antechamber and built the protein topology using amberff99sb provided in gromacs. I get an error "number of coordinates in coordinate file (solv.gro, 95309) does not match topology (topol.top, 95463)" while running the grompp command for adding ions ; gmx grompp -f ions.mdp -c solv.gro -p topol.top -o ions.tpr

It is not including the coordinates for hem and ligand. Although I updated the second line of complex.gro after adding the ligand and hem coordinates. Please find the attached file of complex.gro.

I will be grateful if anyone could suggest some help.