Dear Dr Abdul Main
PCR is the gold standard for pathogen detection while ELISA is enzymatic titration. Due to high sensitivity and specificity.
In terms of accuracy, both ELISA and RT-PCR can be highly accurate when performed correctly. ELISA might be a suitable choice if the primary concern is detecting high viral concentrations quickly and cost-effectively. On the other hand, if you need to detect low viral concentrations or require quantitative data, RT-PCR is the more appropriate option despite its higher cost and complexity.
Kind regards
AB Bayazid
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Both ELISA and RT-PCR are commonly used methods for the detection of Potato virus. However, RT-PCR is generally considered to be more accurate than ELISA. This is because RT-PCR is a molecular technique that can detect the presence of viral RNA, while ELISA detects the presence of viral proteins.
Dear Dr. Abdul Main
RT-PCR is preferable as far as detection of potato virus is concerned because virus levels within potato tubers are normally low and ELISA detection of viruses in these potatoes would be difficult as absorbance values may fall below the set cut-off value. Also, changes that may happen (mutations) at the gene level may influence the immunogenicity of the antigen which is to be detected by ELISA rendering antibodies produced against the previously present virus less effective.
On the other hand, RT-PCR is more sensitive than the ELISA technique. RT-PCR has the ability to detect low viral loads. Also, in RT-PCR, you may detect several viral targets in the same reaction through the use of several primer combinations.
Accuracy, rapidity, and specificity are the key factors for virus detection and therefore RT-PCR is the best method to detect potato virus. However, the only disadvantage of RT-PCR is the cost and technical expertise.
So, if you are involved in routine screening and if you desire to make cost savings, then I would recommend ELISA. Only for borderline cases, you may make use of RT-PCR.
Regards,
Malcolm Nobre