Fluoro-Jade C staining: What is the basic ethanol for?

03 March 2018 1 4K Report

Dear all,

I'm using Fluro-Jade C to stain dead neurons in cryo-sections. As usual, I use a protocol similar to that one described at the merck website: http://www.merckmillipore.com/DE/de/product/Fluoro-Jade-C,MM_NF-AG325

Before placing the slides into 70 % EtOH, slices should be immersed in basic EtOH (1 % NaOH in 80 % EtOH). And I'm wondering what that step is for? Why is it basic EtOH? The FJC dye requires acidic enviroment. If anyone has an explanation, perferably including a reference, I would be happy to hear about it!

Thanks!

Maksim Fomin

Dear Anne-Sophie,

This step is usually done before treatment with potassium permanganate solution. The authors of the paper below say this helps to reduce background staining.

Article Fluoro-Jade C results in high resolution and contrast labeli...

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