Looking for a method to fix an RGD peptide to its integrin receptor prior to Co-IP. Does anyone know of a good method to do so?
I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...
24 February 2021 8,582 1 View
I mistakenly uploaded a copy of the same paper and entered my alternative name. How to delete them out? (I couldn't navigate to the right buttons to do it. So from where to where is what I don't...
07 January 2021 7,694 6 View
I am new into the meta-analysis concept. Having done a little reading and watched videos on YouTube, I understand there are different kinds of meta-analysis. And the type of summary statistics or...
29 December 2020 3,677 3 View
I have a heterogeneous culture of nitrifying bacteria that was originally isolated from the field. It has been kept alive for several months in a bioreactor. I want to pull some of this...
18 December 2020 5,411 4 View
09 November 2020 5,884 5 View
Hello, For one of my projects I would like to test a Live RNA imaging using molecular beacons such as in this paper : Molecular Beacon-based Detection and Isolation of Working-ty... As we...
28 October 2020 9,457 2 View
Hello, I'm new to the bioinformatics world and would like a feedback on which MSA tool to use. I want to find a consensus sequence in about 18000 sequences (from marine biology samples) from...
19 October 2020 2,607 2 View
So I need to store these for 4 weeks at least, before I do a Dyna bead negative T cell isolation on it. What should I store it in FBS? or DMSO and at 4 degrees? or is -20 not good for the cells?
26 July 2020 2,339 3 View
What is the best way to preserve non-sporulating bacteria for further study?
20 May 2020 5,532 5 View
I have a culture of heterogeneous bacteria that will NOT form endospores. This bacteria was originally isolated in nature and is a complex culture of multiple species. I want to be able to...
20 May 2020 8,369 1 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?
28 February 2021 1,254 3 View
23 February 2021 4,014 3 View
I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...
22 February 2021 10,099 5 View
I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...
21 February 2021 4,537 3 View
This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...
21 February 2021 564 3 View
"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...
11 February 2021 6,628 9 View
Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...
11 February 2021 2,513 3 View
Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...
04 February 2021 517 3 View
Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...
03 February 2021 2,032 3 View