Different primers for same gene but different results in expression?

Hey everyone, I am currently doing my doctoral thesis at the department of pathology. I designed two different primers for my gene of interest having two different amplification sizes (130bp and...

17 February 2021 6,684 3 View

By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View

How to address SRMR (0.2 to 0.8) to make model fit?

Dear researchers, I want to update my knowledge regarding data analysis. I will be honored if you can help me to reduce in correcting SRMR in SEM_PLS. All the measures (AVE, CR, VIF, CA, HTMT) are...

09 February 2021 1,702 1 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Are ther ways to quantify / measure the degree of loneliness?

Are there ways to quantify / measure / monitor the degree of loneliness of team members in a Covid-19 lockdown teleworking situation? Team dynamics over a longer period of teleworking and...

16 November 2020 4,212 9 View

Does anybody have experiences with FFF separation of PHB particles?

I am trying to separate suspension of PHB particles, stabilized with PVA. The DLS results show two populations with peak maxima about 150 nm and 380 nm. The Zeta potential was -3 mV (pH 7) across...

21 October 2020 6,551 1 View

What is the impact of the covid-19 pandemic on global warming?

Because of extensive lock up during the pandemic in many countries of the world, notably Europe, USA, Brazil, China and India, there was reduced use of hydrocarbons, especially due to much reduced...

15 October 2020 8,539 23 View

Is there any way to find RUL of bearing with incomplete dataset?

I am interested in finding RUL of bearing, which is currently in operation. I could find papers which uses run to failure datasets, which is not of my interest. Can anyone suggest methods or...

11 October 2020 7,962 7 View

Does anyone know of ontologies of the knowledge covered in US middle school and high school curricula?

For one of our projects we are looking for ontologies of middle and high school topics, such as math, science, history, and others. Is anyone aware of existing ontologies that are available for use?

10 October 2020 2,040 3 View

Multiple-Group Invariance with Categorical Outcomes AND correlated residuals?

Dear all, I have a question relating to the following paper: Dubravka Svetina, Leslie Rutkowski & David Rutkowski (2020) Multiple-Group Invariance with Categorical Outcomes Using Updated...

08 October 2020 3,142 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View