Anyone give the Foreign Examiner detail for Ph.D Microbiology?

I want to know about Foreign examiner details for Thesis evaluation of Ph.D Microbiology. Except Gulf countries.

26 November 2018 9,329 2 View

Cancer diagnosis kit using miRNA probe.....?

I am preparing presentation for my upcoming conference in India. Anyone help me to provide information about difficulties, challenges in using miRNA probe for cancer diagnosis. Thanks in advance

06 February 2017 608 1 View

Has anyone tried modified Azide Azole tech for virus host interaction?

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09 August 2014 5,190 1 View

I am analysing a wing in fluent, getting a very high value of Cl and a very low value of Cd. Why?

I analysed a wing using euler as well as N-S models. I used Spalart Allmaras model for viscous flow. The main problem I am facing is a very high value of coefficient of lift and a very low value...

14 March 2014 1,650 26 View

What is the model in FLUENT that I have to choose in order to analyse induced drag?

I am trying to analyse a 3D wing in FLUENT. I am mainly interested in finding out induced drag. I would like to know the type of model that would give me a correct solution. Inviscid model or any...

05 March 2014 5,346 15 View

SEM and TEM facilities in and around Tamil Nadu, India?

Can you guys list the institutions which provide SEM and TEM service for biological specimens?

28 August 2013 8,117 17 View

How to identify the function of hypothetical proteins?

I have isolated and identified more than 40 hypothetical proteins from E. coli by using MALDI-TOF and LC-MS/MS. Hypothetical proteins are cloned, over expressed and two proteins are characterized...

31 March 2013 3,352 4 View

Inverse peaks in HPLC

Hi I was separating carbohydrates by RP-HPLC. I used to get the solvent peak before the analytes and it was normal. All of a sudden, the solvent peak got inversed. I don't know why? Can anyone...

19 December 2011 3,149 10 View

Friends my question is how to reduce oxygen level in prepared material

Any one give answer

04 October 2011 3,670 4 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

What is the nutrient requirements for E.coli BL21 CodonPlu RIPL strain growing in M9 medium?

Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...

17 February 2021 2,852 2 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the composition of the solid and broth agar to grow Streptomyces from freezed sample? what are extraction steps of products from broth agar?

Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...

26 January 2021 5,006 1 View