Dear Sir. Concerning your issue about the carbohydrate content in animal cells. Carbohydrate composition is determined by acid hydrolysis of a glycoprotein sample to release the individual monosaccharides. After hydrolysis, the monosaccharides (neutral and amino sugars) are derivatized with anthranilic acid (AA, 2-aminobenzoic acid), and then separated from each other and from excess reagent using RP-HPLC. For more details, I think the following below link and the attached file may help you in your analysis:

http://people.umass.edu/~mcclemen/581Carbohydrates.html

Thanks

Dear Prof. Elgailani,

Thank you for very much for your response and for the attached paper. However, it seems that I have conveyed the point of my question incorrectly. The thing is I am now already working with the metabolomics data (GC-MS analysis of cellular extracts, to be exact). I just need to find the numbers on the relative sugar content inside the animal cells, not the techniques of its determination. Since our GC-MS fails to separate the isomeric sugars, the existing information on the sugar content (= just relative numbers), and not only from glycoproteins, might help to better annotate the peaks from my GC-MS chromatograms. Nevertheless, the RP-HPLC chromatogram peaks from the attached paper tell something that I should take into consideration.

Best regards,

Artem

Dear Artem Artiukhov,

I am actually looking for similar information, namely the carbohydrate content of animal cells. By chance, did you find any appropriate reference for this? I would be very happy if you can share it.

With best regards

Jacques

Dear Jacques,

Unfortunately, no, I have not found any detailed information on the matter we are both into and switched to other projects.

Best, Artem

Many thanks for your reply; if I find something, I'll let you know.

Best, Jacques