Can someone please suggest me on a good web-online course in crystallography?

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I would like to know what is the right way to calculate the rate of translation of GFP protein by the ribosome?

I cloned a GFP protein in to three ribosome mutants in order to compare the rate of translation between these three mutants. I used a 96 well plate and a plate reader that measures fluorescence...

31 December 2012 9,946 4 View

create a 105 bp dsDNA

I need to create a 105 bp dsDNA ( by denature the two oligos at 95 degrees for 5 min and than let it cool down slowly). Does anyone know if it will work with such long oligos ?

08 September 2012 8,626 5 View

How to overlay multiple (more than two) )Ligands/ single crystal xrd structures?

Can anyone know how to overlay 3 or more crystal structure/chamical structures by using any software like olex2, mercury or pymol. Mercury has a option to do.. i want to know the step by step process.

16 June 2024 6,871 0 View

Why is sphere not a valid crystal structure ?

If u see nature ex:- honeycomb it is made of hexagon which on heating forms a sphere but y not in crystallography?

26 May 2024 6,604 5 View

How to know the space group of a powder if we only have a XRD pattern of a material and also its not getting matched with any of the standard XRD ?

What's the way out of this problem? Can the single-crystal technique do this task? If yes, What if we also can't make a single crystal of this powder material?

23 May 2024 6,163 2 View

How do we interpret the origin statement ?

Crystallography Doubt_International Table of Crystallography In the space-group number 185 of ITA (see Attachment), how do we interpret the origin statement?

08 May 2024 10,055 0 View

Why are tensile tests of rolled sheets preferably performed on samples parallel to the direction TD and not RD? What makes it more critical?

Why are tensile tests of rolled sheets preferably performed on samples parallel to the direction TD and not RD? What makes it more critical? This preferable orientation is used in papers, and also...

04 May 2024 7,390 1 View

How to Download Xpert High Score Plus V5?

Is there any way to download xpert high score plus v5 or at least v4 by a direct link? There are some links for downloading but they are for v3! I want to download a 2023 COD file and it seems not...

03 May 2024 5,244 5 View

How to fit the complex ITC data?

I've done the ITC experiments to find the calcium-binding affinity of my protein. I used the built-in program, the MicroCal-ITC analysis software to fit the data. The mode that I used to fit is...

28 April 2024 6,223 3 View

How to calculate HKL values without JCPDS card?

I am working on HKL values calculations for Pna21 space type BFO. is there anyone who can recommend a reliable method for the calculations of HKL values without accessing JCPDS cards....?...

13 March 2024 4,942 2 View

CIF FILES and JCPDS cards FOR Manganese (II) acetate, Manganese(III) acetate, Manganese (II/III) phosphate?

Would anyone be able to please provide me with CIF files for Manganese (II) acetate, Manganese(III) acetate, Manganese (II) and MnIII) phosphate. Required for shell fitting couldn't find from...

03 January 2024 1,413 4 View

What to do if I am getting a chunk type of filamentous growth of E.coli BL-21(DE3) cells in growth media?

I work in an enzymology lab focusing towards protein crystallography. We express our gene in pET-28(a) expression vector inside E.coli BL-21(DE3) host leading to protein expression. The problems...

20 December 2023 4,789 3 View