Can I measure molecular biomarkers on amphibians after euthanasia by liquid nitrogen?

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Christoph Leeb

Hi Laurent,

yes this should be possible and I think that it is even recommended as liquid nitrogen stops any kind of metabolism immediately. However, I would suggest to dab the larvae dry with a tissue before putting it in nitrogen. This could be a problem when they are not already dead, so I would still prefer to use MS222.

Best, Christoph

Laurent Boualit

Hi Christoph,

Thanks for your reply. I'm thinking the same but that's would be great to hear something from someone who already tried. That way, if it's not or hardly possible, we could argue for a dispensation from the veterinary services.

Best,

Laurent