What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

What species (or genus even) of crab is this (bivalve using hermit type)?

Two weeks ago I found a peculiar crab in the intertidal zone in SW Florida. when found, the crab was not utilizing a shell. It was similar shaped to porcelain crabs found in our area, but with...

17 January 2021 1,620 4 View

How to extract the time interval between different species visits from camera traps?

How can I use R to extract the time interval between visits of different species from camera trap images? For example, Species A arrives at 07:00 (6 photos, each 1 min apart) Species B arrives at...

14 July 2020 3,155 5 View

In an SDS-PAGE is it necessary to include a sample sample buffer and a protein MW standard?

I am trying to run an SDS-PAGE gel to find a apolipoproteinB-100 which is 550kDa. I found a few papers that referenced Laemmli's SDS-PAGE protocol and it says that the protein sample, the sample...

12 June 2018 3,367 3 View

Is there a general coordinate invariant proof of the divergence theorem using tensor framework?

Does anyone know of a valid reference highlighting the proof of the divergence theorem in a strictly coordinate invariant tensor framework? Kellogg is probably the most frequently sited source,...

17 November 2017 7,230 3 View

Multiple imputation error "no missing values to impute" using SPSS

12 July 2017 5,650 5 View

How can I obtain an OOB error/prediction error when using conditional forest and the variable importance function in the party package?

I know how to obtain the OOB error when using randomForests, but I am using cforest and varimp function in the 'party' package and can't figure out how to get the OOB error/prediction error.

29 March 2016 8,520 2 View

How do I separate fibroblast feeder layer cells from co-cultured adherent tumor cells?

What standard techniques are used to separate cancer cell line cells from fibroblast feeder layer cells after they have been grown in co-culture?

23 September 2015 4,931 7 View

How can I determine the concentration of an aqueous solution of cyanogen?

01 February 2015 8,037 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View