CAn anyone suggest me the best method for synthesis hydroxyapatite other than sol-gel and hydrothermal method?

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Inductive/Deductive Hybrid Thematic Analysis in Qualitative research ?

My research will explore parents' perceptions regarding a specific disease and the research objectives will be derived from a particular theory (Health Belief Model) in a particular cultural...

19 July 2024 6,555 11 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Why were all of my articles removed from ResearchGate?

I have probably misunderstood an email from you. I thought I confirmed my authorship of 16 articles. The result was that a total of 16 articles were removed. I never got a chance to steam....

17 March 2024 5,013 4 View

Concept analysis Rodgers Evolutionary Model - critical appraisal?

Hi there, when undertaking concept analysis using Rodger's model, is it expected to undertake critical appraisal of the included papers? Are there any recommended tools for this? Papers I have...

22 November 2023 1,474 0 View

Could you please recommend websites/resources where i can source for research grants?

Hello! I am recently got approval from my institution to carry out a quasi-experimental study to improve facility-based delivery in my state. I am looking for funding opportunities for this...

11 October 2023 5,667 0 View

Dear Sjogren's Research Community, Is the ESSPRI questionnaire available for other Sjogren's researchers to use please?

Is the ESSPRI questionnaire available for other Sjogren's researchers to use please?

22 August 2023 226 0 View

Is there a replacement method for DNA purification via Cesium Chloride?

I need to purify a cut vector to make it super clean and with no uncut vector. Previously this has been done by Cesium Chloride extraction but we can no longer do that here and I can't find any...

02 April 2023 9,093 0 View

What is the best way to isolate PBMC from whole blood for freezing?

What is the best way to isolate PBMC from blood for freezing? RBC lysis method with ammonium chloride or density gradient with Ficoll? Has anyone done comparison of both methods with cells that...

18 July 2022 3,880 3 View

What research is most recently completed on self-authorship?

Self-Authorship is a part of my research using social emotional learning to understand how the principles incorporated in the Upward Bound Program influenced the academic success and degree...

23 January 2022 3,697 2 View

The incidence and prevalence of rheumatoid arthritis in the world?

I need some articles on the incidence and prevalence of rheumatoid arthritis in the world and also in Denmark.

11 November 2021 5,424 3 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Question about water vapor uptake of metal-organic frameworks?

I'm working on the atmospheric water harvesting, and i synthesized MOF by adding some materials into precursor and changing the conditions. (i followed Hydrothermal method) Actually, if MOF is...

24 July 2024 1,515 0 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,143 3 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

17 July 2024 6,213 4 View

Help. How can I fix my T7 reaction problems?

I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...

16 July 2024 4,604 0 View