Can anyone advise on the best value refrigerated homogenizer for the isolation of thylakoid membranes from multiple samples of microalgae material?

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Anybody with experience with whole plasmid sequencing? Should I worry about plasmid oligomers?

I've sent my plasmids for whole plasmid sequencing using the Oxford nanopore sequencing method. All of them were assembled as monomers, but in the reads (see attached pictures), there are even...

19 June 2024 1,280 5 View

What can be yellow colonies after E. coli TOP10 trnasformation?

Last week, I transformed home-prepared E. coli TOP10 cells. When checking the colonies, I noticed that some of them have yellow color. They did not grow on the randomly, but dependently on the...

16 June 2024 3,272 3 View

How to reduce the number of measurements/iterations needed in deep reinforcement learning?

I'm trying to use reinforcement learning with live EEG measurements. However, just 2000 measurements/iterations take 16.6 minutes to measure and it seems I need at least 10 hours of live...

12 June 2024 2,884 3 View

What can be wrong with this agarose gel?

A friend did PCR and then loaded it on agarose gel. All samples were prepared in the same way; the PCR was identical, with the same template, only primers differed, the same amount loaded on the...

04 June 2024 3,049 3 View

How can I see all points in a 5-95 percentile Boxplot in Graphpad?

Hi! I am trying to do some boxplots in graphpad. I would like to use the 5-95 percentile boxplot option, but if I choose this option I do not know how can I also see the individual values of each...

17 April 2024 6,093 0 View

Why could amplicon sizes appear bigger than they should on agaose gel electrophoresis?

I did PCR of 6 amplicons and when I did agarose gel electrophoresis, they all appear consistently bigger than they should be (e.g. the amplicon should be 4000 bp, but is around band of 5000 bp...

09 April 2024 4,487 5 View

What kind of diatom is this?

I was looking at a sediment sample from a "biosphere" that I´m creating and I came accross this little guy. I´m almost certain that it´s a freshwater diatom but would like to know the specific...

19 March 2024 1,763 10 View

Does somebody have experience with ThermoFisher's Platinum SuperFi II DNA Polymerase?

I just found the Platinum SuperFi II DNA Polymerase, which should simplify PCR protocol as it allows uniform annealing temperature of 60°C, but it should also have very high fidelity; >300×...

17 March 2024 7,686 4 View

Me puede proporcionar iso 9001:2015?

quisiera que me proporciones un sistema de gestion de calidad

15 March 2024 3,491 1 View

Iso 9001:2015?

ME PUEDE PROPORCIONAR INFORMACION SOBRESistema de Gestión de Calidad Basado en la Norma ISO 9001:2015 para el Instituto de Educación Superior Tecnológico

15 March 2024 447 0 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

Hello researchers, how can one get the optical density of a filamentous algae?

i am comparing the growth performances of filamentous and non filamentous forms. in a case where I can not count the cells of the filamentous algae using a hemocytometer, is it okay to do a...

30 July 2024 5,985 2 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

Do you know CUSABIO Biotechnology Scholarships for Students 2024?

I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...

22 July 2024 5,790 1 View

What is the physical meaning of the magnetic scalar potential?

In cases where the rotational of the magnetic field H is zero, we can define this field as the gradient of a scalar function defined as the magnetic scalar potential (similar to the electric...

21 July 2024 9,633 4 View

Where is the stream gradient usually greatest and relationship between the slope of the stream channel and the velocity of the stream?

13 July 2024 5,825 0 View

How do gradient and discharge change in a downstream direction and why are glacial valleys shaped differently from river valleys?

13 July 2024 9,228 3 View

What happens to stream's discharge if gradient of a stream increases & relationship between gradient of a stream & rate of water flow?

What happens to the stream's discharge if the gradient of a stream increases and relationship between the gradient of a stream and the rate of water flow?

13 July 2024 5,085 2 View