hi

i am currently looking at cytoskeleton changes of cell upon applying compression on cells. I have captured images of the stress fibers. I am pretty new to research. What aspect of the stress fiber should i focus on? what are aspects of the stress fiber that i can quantitate to see if compression has any bearing on stress fibers?

- thickness?

- how do i see if there is depolymerization?

- how do i quantitate abundance?

-orientation?