1) I don't want to add any other polymer apart from collagen.
2) Suggest me any good cross linker reagent.
Hello Aurindam Bakshi
You may use 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) in combination. EDC forms amide bonds with hydroxylysine and amines of lysine residues in the presence of NHS by activating the carboxyl groups of aspartic acid or glutamic acid residues, resulting in stable structures.
The references below will be helpful.
https://pubs.aip.org/aip/apm/article/3/1/014902/238345/The-process-of-EDC-NHS-cross-linking-of
https://brieflands.com/articles/jamm-20846
Article The Effect of Crosslinking Agents on the Properties of Type ...
Article Data files for 'Regenerative Biomaterials' article "Effect o...
Best.
To increase the mechanical strength of your pure collagen dressing sheets without adding other polymers, you can use a cross-linking agent. One option is glutaraldehyde, which is very effective but may need careful handling due to potential toxicity. A safer alternative is genipin, a natural cross-linker that strengthens collagen and is biocompatible. Another good option is EDC/NHS, which boosts strength by connecting collagen fibers without leaving extra chemicals behind. Lastly, tannic acid, a plant-based cross-linker, can help with both strength and some antimicrobial benefits.
不同类型的胶原，其分子量、二级结构是不同的，建议选用分子量较大的胶原；
交联一般选择EDC/NHS；
成型方法对于材料的力学强度也有非常大的影响，建议选用热压成型的方法。
@Malcolm Nobre I want to use glutaraldehyde as for scaling up it is quite less expensive as compared to EDC-NHS. Can you please suggest me an optimize ratio for Collagen: Glutaraldehyde and Collagen: D-ribose for an improved scaffold use in the dressing application?
Thank you for your suggestions @JoseAbraham, @Yan-Zheng and @MalcolmNobre. It will be helpful for me if anyone can provide me answer for the above query.
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